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Golgi fragmentation is Rab and SNARE dependent in cellular models of Parkinson's disease.
Rendón, Wilson O; Martínez-Alonso, Emma; Tomás, Mónica; Martínez-Martínez, Narcisa; Martínez-Menárguez, José A.
Afiliação
  • Rendón WO; Department of Cell Biology and Histology, Medical School, University of Murcia, 30100 Murcia, Spain.
Histochem Cell Biol ; 139(5): 671-84, 2013 May.
Article em En | MEDLINE | ID: mdl-23212845
ABSTRACT
Fragmentation of the Golgi ribbon is a common feature of many neurodegenerative diseases but little is known about the causes of this alteration. In Parkinson's disease, it is believed to be the consequence of an ER-Golgi transport imbalance and/or of cytoskeleton alterations. In the present study, we analyze the mechanisms involved in Golgi fragmentation in differentiated PC12 cells treated with 6-hydroxydopamine or methamphetamine as cellular models of Parkinson's disease. Our data demonstrate that Golgi fragmentation precedes and might trigger the aggregation of α-synuclein and the formation of inclusions, alterations in anterograde and retrograde transport between the endoplasmic reticulum and Golgi complex, and cytoskeleton damage. In contrast, fragmentation is directly related with alterations in the levels of Rab1, 2 and 8 and the SNARE protein syntaxin 5. Thus, overexpression of Rab1 and 8 and depletion of Rab2 and syntaxin 5 rescue the Golgi morphology. In conclusion, the homeostasis of a limited number of Rab and SNARE proteins is important for understanding the cytopathology of Parkinson's disease.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença de Parkinson / Proteínas rab de Ligação ao GTP / Proteínas SNARE / Complexo de Golgi / Modelos Biológicos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Histochem Cell Biol Assunto da revista: CITOLOGIA / HISTOCITOQUIMICA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Espanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença de Parkinson / Proteínas rab de Ligação ao GTP / Proteínas SNARE / Complexo de Golgi / Modelos Biológicos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Histochem Cell Biol Assunto da revista: CITOLOGIA / HISTOCITOQUIMICA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Espanha