Functional reconstitution and osmoregulatory properties of the ProU ABC transporter from Escherichia coli.
Mol Membr Biol
; 30(2): 138-48, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-23249124
ABSTRACT
The ATP-binding cassette (ABC) transporter ProU from Escherichia coli translocates a wide range of compatible solutes and contributes to the regulation of cell volume, which is particularly important when the osmolality of the environment fluctuates. We have purified the components of ProU, i.e., the substrate-binding protein ProX, the nucleotide-binding protein ProV and the transmembrane protein ProW, and reconstituted the full transporter complex in liposomes. We engineered a lipid anchor to ProX for surface tethering of this protein to ProVW-containing proteoliposomes. We show that glycine betaine binds to ProX with high-affinity and is transported via ProXVW in an ATP-dependent manner. The activity ProU is salt and anionic lipid-dependent and mimics the ionic strength-gating of transport of the homologous OpuA system.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Transportadores de Cassetes de Ligação de ATP
/
Proteínas de Escherichia coli
/
Proteínas Periplásmicas de Ligação
/
Escherichia coli
Idioma:
En
Revista:
Mol Membr Biol
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Holanda