Testing chemical agents with the cytokinesis-block micronucleus cytome assay.
Folia Biol (Praha)
; 58(5): 215-20, 2012.
Article
em En
| MEDLINE
| ID: mdl-23249641
ABSTRACT
In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action non-mutagens, direct-acting basealtering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimetic chemical, indirect-acting spindle poisons and indirect-acting enzyme inhibitors. We looked at the presence of micronuclei as biomarkers for either the loss of chromosome fragments (clastogen) or the loss of a whole chromosome (aneugen), nucleoplasmic bridges as biomarkers for complex rearrangements (e.g., dicentric chromosomes) and nuclear buds as biomarkers for gene amplification. The cytome assay proved to be a suitable tool to investigate genetic effects of environmental agents and to provide insight into their working mechanisms as all chemicals tested showed the expected response.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testes para Micronúcleos
/
Citocinese
/
Mutagênicos
Limite:
Humans
Idioma:
En
Revista:
Folia Biol (Praha)
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Bélgica