Effects of hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 polymorphisms on fat androstenone level and gene expression in Duroc pigs.
Anim Genet
; 44(5): 592-5, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23581486
ABSTRACT
A high level of androstenone in porcine adipose tissue is a major factor contributing to boar taint. Porcine hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (3ß-HSD, also known as HSD3B1) plays a key role in the hepatic metabolism that catalyzes androstenone to ß-androstenol. Therefore, 3ß-HSD is a candidate gene for boar taint. This study aimed to investigate functional 3ß-HSD polymorphisms in Duroc pigs. We found eight single nucleotide polymorphisms (SNPs) in the full-length porcine 3ß-HSD. Four of the SNPs had restriction enzyme sites, and we genotyped them in 147 uncastrated male Duroc pigs using a polymerase chain reaction-restriction fragment length polymorphism method. Pigs with the GG genotype at the g.165262G>A locus (SNP5) had significantly lower androstenone levels than did those with other genotypes (P = 0.030). SNP5 also was associated with differences in 3ß-HSD mRNA levels pigs with the GG genotype had higher levels than those with other genotypes (P = 0.019). The SNP5 polymorphism could affect the hepatic catabolism of androstenone and consequently impact androstenone accumulation in the adipose tissue. Therefore, SNP5 in the 3ß-HSD of Duroc pigs could be a useful selective marker for decreasing boar taint.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esteroide Isomerases
/
Polimorfismo de Nucleotídeo Único
/
Sus scrofa
Limite:
Animals
Idioma:
En
Revista:
Anim Genet
Assunto da revista:
GENETICA
/
MEDICINA VETERINARIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Coréia do Sul