Distribution of pituitary adenylate cyclase-activating polypeptide (PACAP) in the human testis and in testicular germ cell tumors.
Andrologia
; 46(5): 465-71, 2014 Jun.
Article
em En
| MEDLINE
| ID: mdl-23621806
ABSTRACT
Pituitary adenylate cyclase-activating peptide (PACAP) is a neuropeptide expressed in the central nervous system and peripheral organs. Previous studies revealed the role and distribution of PACAP in the rodent testis, however, its presence in the human testis and in testicular germ cell tumors is not known. We used RT-PCR and immunohistological observations to investigate whether human testicular tissue and testicular germ cell tumors contain PACAP. The mRNAs for PACAP and its receptors were detected in total RNA extracted from human testes. PACAP immunoreactivity was observed in spermatogonia and spermatids from normal testes. In contrast, diffuse PACAP immunopositivity was observed in seminoma tumor cells, while only faint immunoreactivity was observed in embryonal carcinoma cells. Our data suggest that PACAP may play a role in human spermatogenesis and in testicular germ cell tumor development.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Testiculares
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Testículo
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Neoplasias Embrionárias de Células Germinativas
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Polipeptídeo Hipofisário Ativador de Adenilato Ciclase
Limite:
Adult
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Andrologia
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Japão