Specific primer design for the polymerase chain reaction.
Biotechnol Lett
; 35(10): 1541-9, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23794048
ABSTRACT
The design of primers has a major impact on the success of PCR in relation to the specificity and yield of the amplified product. Here, we introduce the applications of PCR as well as the definition and characteristics for PCR primer design. Recent primer design tools based on Primer3, along with several computational intelligence-based primer design methods which have been applied in primer design, are also reviewed. In addition, characteristics of population-based methods used in primer design are discussed in detail.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Primers do DNA
/
Biologia Computacional
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2013
Tipo de documento:
Article