Genetic manipulation of B cells for the isolation of rare therapeutic antibodies from the human repertoire.

Kwakkenbos, Mark J; Bakker, Arjen Q; van Helden, Pauline M; Wagner, Koen; Yasuda, Etsuko; Spits, Hergen; Beaumont, Tim

Kwakkenbos, Mark J; Bakker, Arjen Q; van Helden, Pauline M; Wagner, Koen; Yasuda, Etsuko; Spits, Hergen; Beaumont, Tim.

Afiliação

Kwakkenbos MJ; AIMM Therapeutics, Academic Medical Center, Meibergdreef 59, 1105 BA Amsterdam, The Netherlands. Electronic address: mjkwakkenbos@aimmtherapeutics.com.
Bakker AQ; AIMM Therapeutics, Academic Medical Center, Meibergdreef 59, 1105 BA Amsterdam, The Netherlands. Electronic address: aqbakker@aimmtherapeutics.com.
van Helden PM; AIMM Therapeutics, Academic Medical Center, Meibergdreef 59, 1105 BA Amsterdam, The Netherlands. Electronic address: pmvanhelden@aimmtherapeutics.com.
Wagner K; AIMM Therapeutics, Academic Medical Center, Meibergdreef 59, 1105 BA Amsterdam, The Netherlands. Electronic address: kwagner@aimmtherapeutics.com.
Yasuda E; AIMM Therapeutics, Academic Medical Center, Meibergdreef 59, 1105 BA Amsterdam, The Netherlands. Electronic address: eyasuda@aimmtherapeutics.com.
Spits H; AIMM Therapeutics, Academic Medical Center, Meibergdreef 59, 1105 BA Amsterdam, The Netherlands. Electronic address: hspits@aimmtherapeutics.com.
Beaumont T; AIMM Therapeutics, Academic Medical Center, Meibergdreef 59, 1105 BA Amsterdam, The Netherlands. Electronic address: tbeaumont@aimmtherapeutics.com.

Methods ; 65(1): 38-43, 2014 Jan 01.

Article em En | MEDLINE | ID: mdl-23867338

Assuntos

Anticorpos Monoclonais/isolamento & purificação; Linfócitos B/fisiologia; Animais; Anticorpos Monoclonais/biossíntese; Técnicas de Cultura de Células; Separação Celular; Células Cultivadas; Proteínas de Ligação a DNA/genética; Descoberta de Drogas; Engenharia Genética; Humanos; Proteínas Proto-Oncogênicas c-bcl-6; Receptores de Antígenos de Linfócitos B/genética; Receptores de Antígenos de Linfócitos B/metabolismo; Proteína bcl-X/genética

Palavras-chave

AID; Antibodies; B cell lymphoma-6; B-cell lymphoma-extra large; B-cell receptor; BCL-6; BCR; Bcl-6; Bcl-xL; EBV; Epstein-Barr virus; GALV; Human B cells; IRES; MMLV; RSV; SHM; Transduction; activation-induced cytidine deaminase; gibbon ape leukemia virus; internal ribosomal entry sequence; moloney murine leukemia virus; respiratory syncytial virus; somatic hyper mutation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos B / Anticorpos Monoclonais Limite: Animals / Humans Idioma: En Revista: Methods Assunto da revista: BIOQUIMICA Ano de publicação: 2014 Tipo de documento: Article

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