Meningococcal X polysaccharide quantification by high-performance anion-exchange chromatography using synthetic N-acetylglucosamine-4-phosphate as standard.
Anal Biochem
; 442(2): 259-61, 2013 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-23938776
ABSTRACT
A method for meningococcal X (MenX) polysaccharide quantification by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) is described. The polysaccharide is hydrolyzed by strong acidic treatment, and the peak of glucosamine-4-phosphate (4P-GlcN) is detected and measured after chromatography. In the selected conditions of hydrolysis, 4P-GlcN is the prevalent species formed, with GlcN detected for less than 5% in moles. As standard for the analysis, the monomeric unit of MenX polysaccharide, N-acetylglucosamine-4-phosphate (4P-GlcNAc), was used. This method for MenX quantification is highly selective and sensitive, and it constitutes an important analytical tool for the development of a conjugate vaccine against MenX.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
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Acetilglucosamina
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Cromatografia Líquida de Alta Pressão
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Cromatografia por Troca Iônica
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Glucofosfatos
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Itália