This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Clonagem Molecular
/
Vetores Genéticos
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2013
Tipo de documento:
Article