[Effects of PEG and NaCl stress on the contents of indigotin and indirubin in Isatis indigotica tetraploids].

ABSTRACT

OBJECTIVE: To provide a reference evidence for choosing salt-resistent and drought-resistent varieties of Isatis indigotica Fort.. METHODS: The tissue culture seedlings of different Isatis indigotica tetraploids were used as plant materials. The diploid was used as CK, which were cultured for 30 days. 2 g/L NaCl and 15% PEG-6000 were used as stress treatment respectively for 12 hours, then poured out the solution, the materials were continuously cultured for 7 days. The contents of indirubin and indigotin were determined by HPLC. RESULTS: The contents of indigotin and indirubin of DB3, DB5 and DB12 with PEG treatment and DB2, DB3 and DB12 with NaCl treatment were increased. CONCLUSION: DB3 and DB12 can be further studied as reproduction materials.