Pyk2 and Src mediate signaling to CCL18-induced breast cancer metastasis.
J Cell Biochem
; 115(3): 596-603, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24142406
ABSTRACT
Pyk2 and Src phosphorylation is initiated by CCL18, which promotes breast cancer metastasis via its functional G protein-coupled receptor PITPNM3. However, the function of Pyk2 and Src in CCL18-induced breast cancer metastasis is poorly understood. Quantitative reverse-transcription polymerase chain reactions (qRT-PCRs), Western blot, boyden chamber assay, and adherence assay were performed to delineate the consequences of Pyk2/Src in CCL18-induced breast cancer cells. Co-immunoprecipitation and immunofluorescence were performed to analyze the interaction of proteins. Upon the binding of CCL18 to PITPNM3, Pyk2 translocates from the cytoplasm to the plasma membrane to form a stable complex with PITPNM3, subsequently activating Src kinase. Moreover, upon stimulation with CCL18, Pyk2 and Src become essential for integrin alpha5/beta1 clustering-dependent adherence, migration, and invasion. Pyk2 and Src are important in CCL18-induced breast cancer metastasis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Quinases da Família src
/
Quimiocinas CC
/
Quinase 2 de Adesão Focal
Limite:
Female
/
Humans
Idioma:
En
Revista:
J Cell Biochem
Ano de publicação:
2014
Tipo de documento:
Article