Effect of pH on the recovery of human alpha-fetoprotein from immunosorbent columns. An improved elution procedure.
J Immunol Methods
; 84(1-2): 375-9, 1985 Nov 28.
Article
em En
| MEDLINE
| ID: mdl-2415639
ABSTRACT
A slow decrease in pH was shown to be more efficient in eluting human alpha-fetoprotein bound to Sepharose-immobilized antibodies than a pH shock. With this method, nearly 50% of the antigen absorbed onto the column was recovered in a single peak whereas the yield was 3 times lower in the case of a single elution at pH 2.6. Our result suggests that dissociation of the antigen-antibody complex occurred only within a narrow pH range.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alfa-Fetoproteínas
/
Técnicas de Imunoadsorção
Limite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1985
Tipo de documento:
Article