Multiplex functional bioluminescent reporters using Gaussia luciferase fused to epitope tags in an immunobinding assay.
Methods Mol Biol
; 1098: 231-47, 2014.
Article
em En
| MEDLINE
| ID: mdl-24166381
ABSTRACT
The use of Gaussia luciferase in a multiplex assay can have several advantages over the singleplex method for an experimental setup. Issues such as intersample variability, screening purposes, efficiency, and in vivo applications can be addressed using a multiplex assay. Here we describe a functional reporter multiplex method using Gaussia luciferase fused to epitope tags to identify the different reporters that are expressed. Tag specific antibodies are used to bind and separate the tagged luciferase reporters.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Imuno-Histoquímica
/
Copépodes
/
Luciferases
/
Medições Luminescentes
/
Epitopos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Holanda