Protection from renal fibrosis, putative role of TRIB3 gene silencing.
Exp Mol Pathol
; 96(1): 80-4, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24368111
ABSTRACT
BACKGROUND:
Renal fibrosis is thought to be the common pathway in most cases of chronic kidney disease. Recently, TRIB3 was found to play an important role in progression of cardiac fibrosis in an insulin-resistant state. We investigated whether TRIB3 might participate in the pathogenesis of renal fibrosis in insulin-resistant rats.METHODS:
We randomly separated 40 male Sprague-Dawley into 4 groups for treatment (n = 10 each) control and high-fat diet (HFD) with TRIB3 siRNA adenovirus transfection, vehicle transfection or HFD alone. Insulin resistance markers were measured. Renal tissues were stained with hematoxylin and eosin, Masson's trichrome and periodic acid-Schiff.RESULTS:
Rats with HFD showed insulin resistance and TRIB3 overexpression. Upregulated TRIB3 expression could induce renal fibrosis accompanied by increased phosphorylation of extracellular signal-regulated kinase (ERK). Also, TRIB3 siRNA knockdown could ameliorate renal fibrosis, which was accompanied by decreased phosphorylation of ERK.CONCLUSIONS:
TRIB3 gene silencing can attenuate renal fibrosis for beneficial effect on the development of renal fibrosis in chronic kidney disease in rat.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Fibrose
/
Resistência à Insulina
/
Proteínas Serina-Treonina Quinases
/
Proteínas de Ciclo Celular
/
Inativação Gênica
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Dieta Hiperlipídica
/
Nefropatias
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Exp Mol Pathol
Ano de publicação:
2014
Tipo de documento:
Article