Nitric oxide synthase isoenzyme expression in human oral lichen planus.
J Biol Regul Homeost Agents
; 27(4): 1069-75, 2013.
Article
em En
| MEDLINE
| ID: mdl-24382189
ABSTRACT
The roles of nitric oxide (NO) synthase (NOS) enzyme in pathological mechanisms of the oral cavity are still incompletely understood. The aim of this study was to investigate the expression of the endothelial, neuronal and inducible isoforms of NOS (eNOS, nNOS and iNOS) in oral lichen planus (OLP) development in humans. OLP and healthy oral mucosa biopsies were taken for mRNA and protein analysis of NOS isoenzymes by RT-PCR, western blot and immunohistochemistry. The mRNA and protein levels of eNOS and nNOS were present in all samples, with a significant increase only for eNOS in OLP. The normal oral mucosa exhibited only small amounts of iNOS mRNA and protein, while it showed a significant rise in OLP samples. These results were confirmed by immunohistochemical analysis. Our findings suggest that NO produced by increased eNOS and iNOS expression may have circulatory and immune functions in the development of OLP.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Líquen Plano Bucal
/
Óxido Nítrico Sintase
Limite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Biol Regul Homeost Agents
Assunto da revista:
BIOLOGIA
/
BIOQUIMICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Itália