Synaptic transmission between dissociated adult mammalian neurons and attached synaptic boutons.
Science
; 241(4874): 1810-3, 1988 Sep 30.
Article
em En
| MEDLINE
| ID: mdl-2459774
ABSTRACT
In most studies of synaptic currents in mammalian central neurons, preparations have been used in which synaptic currents are recorded at some distance from the synapse itself. This procedure introduces problems in interpretation of the kinetics and voltage-dependent properties of the synaptic current. These problems have now been overcome by the development of a preparation in which presynaptic vesicle-containing boutons have been coisolated with the soma of individual neurons, thus providing the opportunity to study synaptic currents under conditions of both adequate voltage control and internal ionic perfusion. Spontaneous synaptic currents mediated by gamma-aminobutyric acid and excitatory amino acids were recorded from neurons isolated from a mammalian medial solitary tract nucleus. Calcium- and depolarization-dependent spontaneous currents of several to hundreds of picoamperes occurred with rapid rise times of 0.8 to 3 milliseconds and decays at least ten times as long.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sinapses
/
Transmissão Sináptica
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
1988
Tipo de documento:
Article