Spindle assembly on immobilized chromatin micropatterns.
Methods Enzymol
; 540: 435-48, 2014.
Article
em En
| MEDLINE
| ID: mdl-24630121
ABSTRACT
We describe a method to assemble meiotic spindles on immobilized micropatterns of chromatin built on a first layer of biotinylated BSA deposited by microcontact printing. Such chromatin patterns routinely produce bipolar spindles with a yield of 60%, and offer the possibility to follow spindle assembly dynamics, from the onset of nucleation to the establishment of a quasi steady state. Hundreds of spindles can be recorded in parallel for different experimental conditions. We also describe the semi-automated image analysis pipeline, which is used to analyze the assembly kinetics of spindle arrays, or the final morphological diversity of the spindles.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Proteínas Imobilizadas
/
Fuso Acromático
Limite:
Animals
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Alemanha