Dietary zinc deficiency affects blood linoleic acid: dihomo-γ-linolenic acid (LA:DGLA) ratio; a sensitive physiological marker of zinc status in vivo (Gallus gallus).
Nutrients
; 6(3): 1164-80, 2014 Mar 20.
Article
em En
| MEDLINE
| ID: mdl-24658588
ABSTRACT
Zinc is a vital micronutrient used for over 300 enzymatic reactions and multiple biochemical and structural processes in the body. To date, sensitive and specific biological markers of zinc status are still needed. The aim of this study was to evaluate Gallus gallus as an in vivo model in the context of assessing the sensitivity of a previously unexplored potential zinc biomarker, the erythrocyte linoleic acid dihomo-γ-linolenic acid (LADGLA) ratio. Diets identical in composition were formulated and two groups of birds (n = 12) were randomly separated upon hatching into two diets, Zn⺠(zinc adequate control, 42.3 µg/g zinc), and Znâ» (zinc deficient, 2.5 µg/g zinc). Dietary zinc intake, body weight, serum zinc, and the erythrocyte fatty acid profile were measured weekly. At the conclusion of the study, tissues were collected for gene expression analysis. Body weight, feed consumption, zinc intake, and serum zinc were higher in the Zn⺠control versus Znâ» group (p < 0.05). Hepatic TNF-α, IL-1ß, and IL-6 gene expression were higher in the Zn⺠control group (p < 0.05), and hepatic Δ6 desaturase was significantly higher in the Zn⺠group (p < 0.001). The LADGLA ratio was significantly elevated in the Znâ» group compared to the Zn⺠group (22.6 ± 0.5 and 18.5 ± 0.5, % w/w, respectively, p < 0.001). This study suggests erythrocyte LADGLA is able to differentiate zinc status between zinc adequate and zinc deficient birds, and may be a sensitive biomarker to assess dietary zinc manipulation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Zinco
/
Ácido Linoleico
/
Ácido 8,11,14-Eicosatrienoico
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Nutrients
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos