Monitoring collagen synthesis in fibroblasts using fluorescently labeled tRNA pairs.
J Cell Physiol
; 229(9): 1121-9, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-24676899
ABSTRACT
There is a critical need for techniques that directly monitor protein synthesis within cells isolated from normal and diseased tissue. Fibrotic disease, for which there is no drug treatment, is characterized by the overexpression of collagens. Here, we use a bioinformatics approach to identify a pair of glycine and proline isoacceptor tRNAs as being specific for the decoding of collagen mRNAs, leading to development of a FRET-based approach, dicodon monitoring of protein synthesis (DiCoMPS), that directly monitors the synthesis of collagen. DiCoMPS aimed at detecting collagen synthesis will be helpful in identifying novel anti-fibrotic compounds in cells derived from patients with fibrosis of any etiology, and, suitably adapted, should be widely applicable in monitoring the synthesis of other proteins in cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência de Glicina
/
RNA de Transferência de Prolina
/
Colágeno
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Microscopia Confocal
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Transferência Ressonante de Energia de Fluorescência
/
Fibroblastos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Cell Physiol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China