Induction of prostacyclin receptor expression in human erythroleukemia cells.
FEBS Lett
; 255(1): 172-4, 1989 Sep 11.
Article
em En
| MEDLINE
| ID: mdl-2477277
ABSTRACT
We have identified both high-affinity (KD = 36 +/- 3 nM) and low-affinity (KD = 2.1 +/- 0.8 microM) prostacyclin (PGI2)-receptor sites on human erythroleukemia (HEL) cells using the radiolabelled prostacyclin analogue. [3H]iloprost. The addition of the phorbol ester, TPA, to the culture medium caused a 5-10-fold increase in the number of both the low- and the high-affinity sites, without any change in their affinity constants. Iloprost stimulated HEL cell membrane adenylate cyclase activity 5-fold. This stimulation was potentiated in the presence of GTP, indicating a conventional PGI2 receptor-G2-adenylate cyclase system. HEL cells represent a source of prostacyclin receptor mRNA which may be of value in expression cloning of this receptor.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ésteres de Forbol
/
Leucemia Eritroblástica Aguda
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1989
Tipo de documento:
Article