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A DNA microarray-based assay to detect dual infection with two dengue virus serotypes.
Díaz-Badillo, Alvaro; Muñoz, María de Lourdes; Perez-Ramirez, Gerardo; Altuzar, Victor; Burgueño, Juan; Mendoza-Alvarez, Julio G; Martínez-Muñoz, Jorge P; Cisneros, Alejandro; Navarrete-Espinosa, Joel; Sanchez-Sinencio, Feliciano.
Afiliação
  • Díaz-Badillo A; Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico. alvaro@cinvestav.mx.
  • Muñoz Mde L; Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico. lmunoz@cinvestav.mx.
  • Perez-Ramirez G; Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico. gperezr@cinvestav.mx.
  • Altuzar V; Centro de Investigación en Micro y Nanotecnología (MICRONA), Universidad Veracruzana, Boca del Rio, Veracruz, 94294, Mexico. valtuzar@gmail.com.
  • Burgueño J; Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT), Texcoco, Estado de Mexico, 56237, Mexico. jabfpc@yahoo.com.
  • Mendoza-Alvarez JG; Department of Physics, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico. juliogma1@gmail.com.
  • Martínez-Muñoz JP; Laboratorio Estatal de Salud Pública de Oaxaca, Servicios de Salud de Oaxaca, Oaxaca, 71257, Mexico. monterrey2bios@hotmail.com.
  • Cisneros A; Escuela de Medicina Veterinaria y Zootecnia, Universidad Autónoma Benito Juarez de Oaxaca, 68120, Oaxaca, Mexico. alecis@msn.com.
  • Navarrete-Espinosa J; División de Epidemiologia, Coordinación de Programas Integrados de Salud, Instituto Mexicano del Seguro Social, Mexico D.F., 06700, Mexico. joel.navarrete@imss.gob.mx.
  • Sanchez-Sinencio F; Department of Physics, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico. fsanchez@fis.cinvestav.mx.
Sensors (Basel) ; 14(5): 7580-601, 2014 Apr 25.
Article em En | MEDLINE | ID: mdl-24776933
ABSTRACT
Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Viral / Técnicas Biossensoriais / Análise de Sequência com Séries de Oligonucleotídeos / Dengue / Vírus da Dengue Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Sensors (Basel) Ano de publicação: 2014 Tipo de documento: Article País de afiliação: México

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Viral / Técnicas Biossensoriais / Análise de Sequência com Séries de Oligonucleotídeos / Dengue / Vírus da Dengue Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Sensors (Basel) Ano de publicação: 2014 Tipo de documento: Article País de afiliação: México