A pertussis toxin-sensitive GTP-binding protein plays a role in the G0-G1 transition of rat hepatocytes following establishment in primary culture.
FEBS Lett
; 245(1-2): 117-21, 1989 Mar 13.
Article
em En
| MEDLINE
| ID: mdl-2494069
ABSTRACT
Acute spontaneous c-myc gene expression and sustained increase of a GTP-binding protein(s) (G-protein) which is sensitive to islet-activating protein (IAP), pertussis toxin, occurred early during primary culture of adult rat hepatocytes. Following these earlier events, DNA synthesis was demonstrated in response to EGF and insulin. Addition of IAP immediately after plating of primary cultures inhibited c-myc expression and the hormone-induced DNA synthesis. Addition at 24 h or later following cell inoculation, however, produced only weak effects on DNA synthesis, even though the IAP-sensitive G-proteins were completely inactivated. We conclude that the IAP-sensitive G-protein(s) plays a role in the earlier process(es) of the G0-G1 transition, which is essential for the initiation of growth factor-dependent DNA synthesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Virulência de Bordetella
/
Proteínas de Ligação ao GTP
/
Toxina Pertussis
/
Interfase
/
Fígado
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Japão