Lactulose: mannitol diagnostic test by HPLC and LC-MSMS platforms: considerations for field studies of intestinal barrier function and environmental enteropathy.
J Pediatr Gastroenterol Nutr
; 59(4): 544-50, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-24941958
ABSTRACT
OBJECTIVES:
The lactulosemannitol (LM) diagnostic test is frequently used in field studies of environmental enteropathy (EE); however, heterogeneity in test administration and disaccharide measurement has limited the comparison of results between studies and populations. We aim to assess the agreement between LM measurement between high-performance liquid chromatography with pulsed amperometric detection (HPLC-PAD) and liquid chromatography-tandem mass spectrometry (LC-MSMS) platforms.METHODS:
The LM test was administered in a cohort of Peruvian infants considered at risk for EE. A total of 100 samples were tested for lactulose and mannitol at 3 independent laboratories 1 running an HPLC-PAD platform and 2 running LC-MSMS platforms. Agreement between the platforms was estimated.RESULTS:
The Spearman correlation between the 2 LC-MSMS platforms was high (ρ ≥ 0.89) for mannitol, lactulose, and the LM ratio. The correlation between the HPLC-PAD platform and LC-MSMS platform was ρ = 0.95 for mannitol, ρ = 0.70 for lactulose, and ρ = 0.43 for the LM ratio. In addition, the HPLC-PAD platform overestimated the lowest disaccharide concentrations to the greatest degree.CONCLUSIONS:
Given the large analyte concentration range, the improved accuracy of LC-MSMS has important consequences for the assessment of lactulose and mannitol following oral administration in populations at risk for EE. We recommend that researchers wishing to implement a dual-sugar test as part of a study of EE use an LC-MSMS platform to optimize the accuracy of results and increase comparability between studies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Técnicas de Diagnóstico do Sistema Digestório
/
Espectrometria de Massas em Tandem
/
Absorção Intestinal
/
Enteropatias
/
Lactulose
/
Manitol
Tipo de estudo:
Diagnostic_studies
Limite:
Female
/
Humans
/
Infant
/
Male
País/Região como assunto:
America do sul
/
Peru
Idioma:
En
Revista:
J Pediatr Gastroenterol Nutr
Ano de publicação:
2014
Tipo de documento:
Article