Effect of post-solubilization conditions on the yield and efficiency of recombinant streptokinase purification at large-scale.

Beiroti, Ahmad; Kavianpour, Alireza; Sepahi, Mina; Arsalani, Firooz; Tavakoli Zaniani, Parinaz; Jalalirad, Reza

Beiroti, Ahmad; Kavianpour, Alireza; Sepahi, Mina; Arsalani, Firooz; Tavakoli Zaniani, Parinaz; Jalalirad, Reza.

Afiliação

Beiroti A; Department of Streptokinase and Interferon Production, Production & Research Complex, Pasteur Institute of Iran, Tehran-Karaj Highway, Postal Code: 31599, Karaj, Iran.
Kavianpour A; Department of Streptokinase and Interferon Production, Production & Research Complex, Pasteur Institute of Iran, Tehran-Karaj Highway, Postal Code: 31599, Karaj, Iran.
Sepahi M; Department of Streptokinase and Interferon Production, Production & Research Complex, Pasteur Institute of Iran, Tehran-Karaj Highway, Postal Code: 31599, Karaj, Iran.
Arsalani F; Department of Streptokinase and Interferon Production, Production & Research Complex, Pasteur Institute of Iran, Tehran-Karaj Highway, Postal Code: 31599, Karaj, Iran.
Tavakoli Zaniani P; Department of Streptokinase and Interferon Production, Production & Research Complex, Pasteur Institute of Iran, Tehran-Karaj Highway, Postal Code: 31599, Karaj, Iran.
Jalalirad R; Department of Streptokinase and Interferon Production, Production & Research Complex, Pasteur Institute of Iran, Tehran-Karaj Highway, Postal Code: 31599, Karaj, Iran. Electronic address: rjalalirad@gmail.com.

Protein Expr Purif ; 101: 127-32, 2014 Sep.

Article em En | MEDLINE | ID: mdl-24954109

Assuntos

Fibrinolíticos/isolamento & purificação; Corpos de Inclusão/química; Proteínas Recombinantes/isolamento & purificação; Estreptoquinase/isolamento & purificação; Cromatografia em Gel; Cromatografia por Troca Iônica; Eletroforese em Gel de Poliacrilamida; Fibrinólise/efeitos dos fármacos; Fibrinolíticos/farmacocinética; Concentração de Íons de Hidrogênio; Interações Hidrofóbicas e Hidrofílicas; Infarto do Miocárdio/tratamento farmacológico; Proteínas Recombinantes/genética; Proteínas Recombinantes/farmacocinética; Estreptoquinase/genética; Estreptoquinase/farmacocinética

Palavras-chave

Chromatographic purification; Downstream processing; Inclusion bodies; Post-solubilization; Recombinant streptokinase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estreptoquinase / Proteínas Recombinantes / Corpos de Inclusão / Fibrinolíticos Idioma: En Revista: Protein Expr Purif Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Irã

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