Methamphetamine modulates glutamatergic synaptic transmission in rat primary cultured hippocampal neurons.
Brain Res
; 1582: 1-11, 2014 Sep 25.
Article
em En
| MEDLINE
| ID: mdl-25091639
ABSTRACT
Methamphetamine (METH) is a psychostimulant drug. Abuse of METH produces long-term behavioral changes including behavioral, sensitization, tolerance, and dependence. It induces neurotoxic effects in several areas of the brain via enhancing dopamine (DA) level abnormally, which may cause a secondary release of glutamate (GLU). However, repeated administration of METH still increases release of GLU even when dopamine content in tissue is significantly depleted. It implies that some other mechanisms are likely to involve in METH-induced GLU release. The goal of this study was to observe METH affected glutamatergic synaptic transmission in rat primary cultured hippocampal neurons and to explore the mechanism of METH modulated GLU release. Using whole-cell patch-clamp recordings, we found that METH (0.1-50.0µM) increased the frequency of spontaneous excitatory postsynaptic currents (sEPSCs) and miniature excitatory postsynaptic currents (mEPSCs). However, METH decreased the frequency of sEPSCs and mEPSCs at high concentration of 100µM. The postsynaptic NMDA receptor currents and P/Q-type calcium channel were not affected by the use of METH (10,100µM). METH did not present visible effect on N-type Ca(2+) channel current at the concentration lower than 50.0µM, but it was inhibited by use of METH at a 100µM. The effect of METH on glutamatergic synaptic transmission was not revered by pretreated with DA receptor antagonist SCH23390. These results suggest that METH directly modulated presynaptic GLU release at a different concentration, while dopaminergic system was not involved in METH modulated release of GLU in rat primary cultured hippocampal neurons.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transmissão Sináptica
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Ácido Glutâmico
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Hipocampo
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Estimulantes do Sistema Nervoso Central
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Metanfetamina
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Neurônios
Limite:
Animals
Idioma:
En
Revista:
Brain Res
Ano de publicação:
2014
Tipo de documento:
Article