Your browser doesn't support javascript.
loading
Core-binding factor subunit beta is not required for non-primate lentiviral Vif-mediated APOBEC3 degradation.
Ai, Youwei; Zhu, Dantong; Wang, Cuihui; Su, Chao; Ma, Jian; Ma, Jianzhang; Wang, Xiaojun.
Afiliação
  • Ai Y; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China College of Wildlife Resources, Northeast Forestry University, Harbin, China.
  • Zhu D; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China College of Wildlife Resources, Northeast Forestry University, Harbin, China.
  • Wang C; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
  • Su C; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
  • Ma J; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
  • Ma J; College of Wildlife Resources, Northeast Forestry University, Harbin, China jianzhangma@163.com xjw@hvri.ac.cn.
  • Wang X; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China jianzhangma@163.com xjw@hvri.ac.cn.
J Virol ; 88(20): 12112-22, 2014 Oct.
Article em En | MEDLINE | ID: mdl-25122780
Viral infectivity factor (Vif) is required for lentivirus fitness and pathogenicity, except in equine infectious anemia virus (EIAV). Vif enhances viral infectivity by a Cullin5-Elongin B/C E3 complex to inactivate the host restriction factor APOBEC3. Core-binding factor subunit beta (CBF-ß) is a cell factor that was recently shown to be important for the primate lentiviral Vif function. Non-primate lentiviral Vif also degrades APOBEC3 through the proteasome pathway. However, it is unclear whether CBF-ß is required for the non-primate lentiviral Vif function. In this study, we demonstrated that the Vifs of non-primate lentiviruses, including feline immunodeficiency virus (FIV), bovine immunodeficiency virus (BIV), caprine arthritis encephalitis virus (CAEV), and maedi-visna virus (MVV), do not interact with CBF-ß. In addition, CBF-ß did not promote the stability of FIV, BIV, CAEV, and MVV Vifs. Furthermore, CBF-ß silencing or overexpression did not affect non-primate lentiviral Vif-mediated APOBEC3 degradation. Our results suggest that non-primate lentiviral Vif induces APOBEC3 degradation through a different mechanism than primate lentiviral Vif. Importance: The APOBEC3 protein family members are host restriction factors that block retrovirus replication. Vif, an accessory protein of lentivirus, degrades APOBEC3 to rescue viral infectivity by forming Cullin5-Elongin B/C-based E3 complex. CBF-ß was proved to be a novel regulator of primate lentiviral Vif function. In this study, we found that CBF-ß knockdown or overexpression did not affect FIV Vif's function, which induced polyubiquitination and degradation of APOBEC3 by recruiting the E3 complex in a manner similar to that of HIV-1 Vif. We also showed that other non-primate lentiviral Vifs did not require CBF-ß to degrade APOBEC3. CBF-ß did not interact with non-primate lentiviral Vifs or promote their stability. These results suggest that a different mechanism exists for the Vif-APOBEC interaction and that non-primates are not suitable animal models for exploring pharmacological interventions that disrupt Vif-CBF-ß interaction.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Produtos do Gene vif / Lentivirus / Citosina Desaminase / Subunidade beta de Fator de Ligação ao Core Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Virol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Produtos do Gene vif / Lentivirus / Citosina Desaminase / Subunidade beta de Fator de Ligação ao Core Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Virol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China