Molecular characterization of porcine Siglec-10 and analysis of its expression in blood and tissues.
Dev Comp Immunol
; 48(1): 116-23, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25280627
ABSTRACT
Siglecs are sialic acid binding Ig-like proteins involved in the control of leukocyte responses. In this study we describe the characterization of a porcine orthologue of Siglec-10. A cDNA clone was obtained from a porcine library which encodes a protein with sequence homology to human Siglec-10. This cDNA codes for a type I transmembrane protein containing four Ig-like domains, a transmembrane region, and a cytoplasmic tail with three tyrosine-based motifs, including a membrane-proximal Grb2-binding motif, and two ITIM motifs. When expressed on transfected cells, porcine Siglec-10 was able to bind red blood cells in a sialic acid-dependent manner. Monoclonal antibodies were developed against this protein and used to examine its cell and tissue distribution in the pig. Siglec-10 was found to be expressed on blood B cells and B cell areas of the spleen and lymph nodes. A weak expression was also detected on monocytes.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Eritrócitos
/
Lectinas Semelhantes a Imunoglobulina de Ligação ao Ácido Siálico
Limite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Espanha