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Spontaneous dormancy of metastatic breast cancer cells in an all human liver microphysiologic system.
Wheeler, S E; Clark, A M; Taylor, D P; Young, C L; Pillai, V C; Stolz, D B; Venkataramanan, R; Lauffenburger, D; Griffith, L; Wells, A.
Afiliação
  • Wheeler SE; Department of Pathology, University of Pittsburgh, S711 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA, USA.
  • Clark AM; Department of Pathology, University of Pittsburgh, S711 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA, USA.
  • Taylor DP; 1] Department of Pathology, University of Pittsburgh, S711 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA, USA [2] Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
  • Young CL; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Pillai VC; Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, PA, USA.
  • Stolz DB; 1] Department of Pathology, University of Pittsburgh, S711 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA, USA [2] Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA [3] McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA [4] University
  • Venkataramanan R; 1] Department of Pathology, University of Pittsburgh, S711 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA, USA [2] Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, PA, USA.
  • Lauffenburger D; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Griffith L; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Wells A; 1] Department of Pathology, University of Pittsburgh, S711 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA, USA [2] Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA [3] McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA [4] Universi
Br J Cancer ; 111(12): 2342-50, 2014 Dec 09.
Article em En | MEDLINE | ID: mdl-25314052
ABSTRACT

BACKGROUND:

Metastatic outgrowth in breast cancer can occur years after a seeming cure. Existing model systems of dormancy are limited as they do not recapitulate human metastatic dormancy without exogenous manipulations and are unable to query early events of micrometastases.

METHODS:

Here, we describe a human ex vivo hepatic microphysiologic system. The system is established with fresh human hepatocytes and non-parenchymal cells (NPCs) creating a microenvironment into which breast cancer cells (MCF7 and MDA-MB-231) are added.

RESULTS:

The hepatic tissue maintains function through 15 days as verified by liver-specific protein production and drug metabolism assays. The NPCs form an integral part of the hepatic niche, demonstrated within the system through their participation in differential signalling cascades and cancer cell outcomes. Breast cancer cells intercalate into the hepatic niche without interfering with hepatocyte function. Examination of cancer cells demonstrated that a significant subset enter a quiescent state of dormancy as shown by lack of cell cycling (EdU(-) or Ki67(-)). The presence of NPCs altered the cancer cell fraction entering quiescence, and lead to differential cytokine profiles in the microenvironment effluent.

CONCLUSIONS:

These findings establish the liver microphysiologic system as a relevant model for the study of breast cancer metastases and entry into dormancy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Neoplasias Hepáticas Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: Br J Cancer Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Neoplasias Hepáticas Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: Br J Cancer Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Estados Unidos