Determination of the molecular size of the 5-HT3 receptor binding site by radiation inactivation.
Eur J Pharmacol
; 172(6): 497-500, 1989 Dec 05.
Article
em En
| MEDLINE
| ID: mdl-2533081
ABSTRACT
The radiation inactivation technique has been used to estimate the molecular size of the 5-HT3 receptor binding site labelled by [3H]zacopride, in comparison with that of the 5-HT1A receptor binding site labelled by [3H]8-OH-DPAT, in rat cortical membranes. The calculated molecular weight of the 5-HT3 site 35.4 +/- 2.2 kDa (mean +/- S.E.M., n = 4) was significantly less than that of the 5-HT1A site 62.9 +/- 1.8 kDa (mean +/- S.E.M., n = 4) and of other 5-HT1 and 5-HT2 receptors of the G-protein coupled family. These data further support that the 5-HT3 receptor is not coupled to G-proteins in the rat brain.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Serotonina
/
Compostos Bicíclicos Heterocíclicos com Pontes
Limite:
Animals
Idioma:
En
Revista:
Eur J Pharmacol
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
França