[3H]AHN 086 acylates peripheral benzodiazepine receptors in the rat pineal gland.
FEBS Lett
; 244(2): 263-7, 1989 Feb 27.
Article
em En
| MEDLINE
| ID: mdl-2537753
ABSTRACT
AHN 086, an isothiocyanato derivative of Ro 5-4864 (4'-chlorodiazepam), inhibits radioligand binding to peripheral benzodiazepine receptors with characteristics of an irreversible (acylating) ligand. We now report that [3H]AHN 086 labels a approximately 30 kDa protein in the rat pineal gland determined by both SDS-polyacrylamide gel electrophoresis and gel filtration high-performance liquid chromatography of digitonin-solubilized membranes. Specific incorporation of [3H]AHN 086 into this protein was inhibited by preincubating membranes with excess AHN 086. Moreover, significant specific binding of [3H]AHN 086 was not observed in either bovine pineal gland (which does not possess high-affinity binding sites for Ro 5-4864) or ovalbumin. These findings suggest that the approximately 30 kDa protein labeled by [3H]AHN 086 in rat pineal gland is associated with peripheral benzodiazepine receptors in this tissue.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glândula Pineal
/
Benzodiazepinonas
/
Receptores de GABA-A
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1989
Tipo de documento:
Article