Unusual DNA structures at the integration site of an HIV provirus.
Biochem Biophys Res Commun
; 161(1): 134-42, 1989 May 30.
Article
em En
| MEDLINE
| ID: mdl-2543406
ABSTRACT
Supercoiled pHXBc2 DNA (containing the genome of the human immunodeficiency virus type 1 and human sequences) migrated more slowly than linear DNA in native and ethidium bromide agarose gel electrophoresis at 4.5 volts/cm, suggesting the presence of unusual DNA structures. S1 nuclease analysis of pHXBc2 revealed two S1 hypersensitive sites. Site I was located within a 25 bp direct repeat in host DNA 0.6 kB upstream from the 5' LTR. Site II was mapped 0.2 kB upstream from the vif gene start site. Sequence analysis showed that Site I sequences could assume different unusual DNA structures, whereas sequences at Site II could assume either slipped or H-DNA forms. Unusual DNA structures in host DNA may be associated with active chromatin regions and may favor proviral integration.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Super-Helicoidal
/
HIV-1
/
Provírus
/
Genes Virais
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Marrocos