The dengue virus conceals double-stranded RNA in the intracellular membrane to escape from an interferon response.
Sci Rep
; 4: 7395, 2014 Dec 10.
Article
em En
| MEDLINE
| ID: mdl-25491663
ABSTRACT
The dengue virus (DENV) circulates between humans and mosquitoes and requires no other mammals or birds for its maintenance in nature. The virus is well-adapted to humans, as reflected by high-level viraemia in patients. To investigate its high adaptability, the DENV induction of host type-I interferon (IFN) was assessed in vitro in human-derived HeLa cells and compared with that induced by the Japanese encephalitis virus (JEV), a closely related arbovirus that generally exhibits low viraemia in humans. A sustained viral spread with a poor IFN induction was observed in the DENV-infected cells, whereas the JEV infection resulted in a self-limiting and abortive infection with a high IFN induction. There was no difference between DENV and JEV double-stranded RNA (dsRNA) as IFN inducers. Instead, the dsRNA was poorly exposed in the cytosol as late as 48 h post-infection (p.i.), despite the high level of DENV replication in the infected cells. In contrast, the JEV-derived dsRNA appeared in the cytosol as early as 24 h p.i. Our results provided evidence for the first time in DENV, that concealing dsRNA in the intracellular membrane diminishes the effect of the host defence mechanism, a strategy that differs from an active suppression of IFN activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA de Cadeia Dupla
/
RNA Viral
/
Interferon Tipo I
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Vírus da Dengue
/
Evasão da Resposta Imune
/
Membranas Intracelulares
Limite:
Humans
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Japão