GC-C-IRMS in routine doping control practice: 3 years of drug testing data, quality control and evolution of the method.
Anal Bioanal Chem
; 407(15): 4397-409, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-25503937
ABSTRACT
In order to detect the misuse of endogenous anabolic steroids, doping control laboratories require methods that allow differentiation between endogenous steroids and their synthetic copies. Gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) is capable of measuring the carbon isotope ratio of urinary steroids and this allows differentiation between both. GC-C-IRMS and its application to doping control has evolved a lot during the last decade and so have the World Anti-Doping Agency (WADA) technical documents that describe how GC-C-IRMS should be applied. Especially the WADA technical document of 2014 introduced a number of obligatory quality controls and a fixed methodology that should be used by all the doping control laboratories. This document imposed more uniform methods between the laboratories in order to decrease the interlaboratory standard deviation and acquire similar results for the analysis of the same urine samples. In this paper, 3 years of drug testing data of our GC-C-IRMS method in routine doping control practice is described, with an emphasis on the new WADA technical document and its implementation. Useful data for other doping control laboratories is presented focussing on general method setup, quality control and data collected from routine samples. The paper concentrates on how IRMS results shift or remain similar by switching to the 2014 WADA technical document and gives insight in a straightforward approach to calculate the measurement uncertainty.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esteroides
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Detecção do Abuso de Substâncias
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Anabolizantes
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Cromatografia Gasosa-Espectrometria de Massas
Tipo de estudo:
Evaluation_studies
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Anal Bioanal Chem
Ano de publicação:
2015
Tipo de documento:
Article