A combined light sheet fluorescence and differential interference contrast microscope for live imaging of multicellular specimens.
J Microsc
; 258(2): 105-12, 2015 May.
Article
em En
| MEDLINE
| ID: mdl-25611324
ABSTRACT
We describe a microscope capable of both light sheet fluorescence microscopy and differential interference contrast microscopy (DICM). The two imaging modes, which to the best of our knowledge have not previously been combined, are complementary light sheet fluorescence microscopy provides three-dimensional imaging of fluorescently labelled components of multicellular systems with high speed, large fields of view, and low phototoxicity, whereas differential interference contrast microscopy reveals the unlabelled neighbourhood of tissues, organs, and other structures with high contrast and inherent optical sectioning. Use of a single Nomarski prism for differential interference contrast microscopy and a shared detection path for both imaging modes enables simple integration of the two techniques in one custom microscope. We provide several examples of the utility of the resulting instrument, focusing especially on the digestive tract of the larval zebrafish, revealing in this complex and heterogeneous environment anatomical features, the behaviour of commensal microbes, immune cell motions, and more.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imageamento Tridimensional
/
Microscopia Intravital
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Microscopia de Fluorescência
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Microscopia de Interferência
Limite:
Animals
Idioma:
En
Revista:
J Microsc
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos