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SCF/c-kit signaling is required in 12-O-tetradecanoylphorbol-13-acetate-induced migration and differentiation of hair follicle melanocytes for epidermal pigmentation.
Qiu, Weiming; Yang, Ke; Lei, Mingxing; Yan, Hongtao; Tang, Hui; Bai, Xiufeng; Yang, Guihong; Lian, Xiaohua; Wu, Jinjin.
Afiliação
  • Qiu W; Department of Dermatology, Daping Hospital, The Third Military Medical University, Chongqing, 400042, China.
Cell Tissue Res ; 360(2): 333-46, 2015 May.
Article em En | MEDLINE | ID: mdl-25727244
ABSTRACT
Hair follicle melanocyte stem cells (McSCs) are responsible for hair pigmentation and also function as a major melanocyte reservoir for epidermal pigmentation. However, the molecular mechanism promoting McSCs for epidermal pigmentation remains elusive. 12-O-tetradecanoylphorbol-13-acetate (TPA) mimics key signaling involved in melanocyte growth, migration and differentiation. We therefore investigated the molecular basis for the contribution of hair follicle McSCs to epidermal pigmentation using the TPA induction model. We found that repetitive TPA treatment of female C57BL/6 mouse dorsal skin induced epidermal pigmentation by increasing the number of epidermal melanocytes. Particularly, TPA treatment induced McSCs to initiate proliferation, exit the stem cell niche and differentiate. We also demonstrated that TPA promotes melanoblast migration and differentiation in vitro. At the molecular level, TPA treatment induced robust expression of stem cell factor (SCF) in keratinocytes and c-kit in melanoblasts and melanocytes. Administration of ACK2, a neutralizing antibody against the Kit receptor, suppressed mouse epidermal pigmentation, decreased the number of epidermal melanocytes, and inhibited melanoblast migration. Taken together, our data demonstrate that TPA promotes the expansion, migration and differentiation of hair follicle McSCs for mouse epidermal pigmentation. SCF/c-kit signaling was required for TPA-induced migration and differentiation of hair follicle melanocytes. Our findings may provide an excellent model to investigate the signaling mechanisms regulating epidermal pigmentation from mouse hair follicle McSCs, and a potential therapeutic option for skin pigmentation disorders.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pigmentação da Pele / Acetato de Tetradecanoilforbol / Carcinógenos / Transdução de Sinais / Diferenciação Celular / Movimento Celular / Fator de Células-Tronco / Proteínas Proto-Oncogênicas c-kit / Folículo Piloso / Melanócitos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Cell Tissue Res Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pigmentação da Pele / Acetato de Tetradecanoilforbol / Carcinógenos / Transdução de Sinais / Diferenciação Celular / Movimento Celular / Fator de Células-Tronco / Proteínas Proto-Oncogênicas c-kit / Folículo Piloso / Melanócitos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Cell Tissue Res Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China