A rapid protocol for the purification of mitochondrial DNA suitable for studying restriction fragment length polymorphisms.
Gene
; 83(1): 169-72, 1989 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-2574128
ABSTRACT
When analyzing mitochondrial DNA (mtDNA) from various normal and malignant human tissues, it became necessary to enhance mtDNA isolation for improved yields and quality. The method described here consists of rapid and simple-to-perform steps, avoiding complicated instrumentation. It was designed for preparation of undegraded mtDNA and is highly useful when limited amounts of tissues, cells and unique biopsies of tumors (fresh or frozen) are available. The resulting mtDNA is sufficiently pure for restriction analysis, subcloning, labeling and various types of hybridization. Using Sau3A and MspI, restriction analysis revealed new restriction-fragment length polymorphisms for Caucasians, independent of the DNA source, and hence excluding tissue-specific DNA modifications.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo de Fragmento de Restrição
/
DNA Mitocondrial
Limite:
Humans
Idioma:
En
Revista:
Gene
Ano de publicação:
1989
Tipo de documento:
Article