Analysis of a temperature-sensitive mutation in Uba1: Effects of the click reaction on subsequent immunolabeling of proteins involved in DNA replication.

Sugaya, Kimihiko; Ishihara, Yoshie; Inoue, Sonoe

Sugaya, Kimihiko; Ishihara, Yoshie; Inoue, Sonoe.

Afiliação

Sugaya K; Research Center for Radiation Protection and Fukushima Project Headquarters, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
Ishihara Y; Research Center for Radiation Protection and Fukushima Project Headquarters, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
Inoue S; Research Center for Radiation Protection and Fukushima Project Headquarters, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.

FEBS Open Bio ; 5: 167-74, 2015.

Article em En | MEDLINE | ID: mdl-25834782

Palavras-chave

BrdU, 5-bromo-2'-deoxyuridine; Chromosome instability; CldU, 5-chloro-2'-deoxyuridine; Click chemistry; DIG-dUTP, digoxigenin-dUTP; E1, ubiquitin activating enzyme; E2, ubiquitin conjugating enzyme; E3, ubiquitin ligase; EdU, 5-ethynyl-2'-deoxyuridine; IdU, 5-iodo-2'-deoxyuridine; MCM7, mini-chromosome maintenance protein 7; PCNA, proliferating cell nuclear antigen; PFA, paraformaldehyde; Replication; Temperature-sensitive mutation; Ubiquitination; ts, temperature-sensitive

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: FEBS Open Bio Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Japão

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