Identification of novel phosphatidic acid-binding proteins in the rat brain.
Neurosci Lett
; 595: 108-13, 2015 May 19.
Article
em En
| MEDLINE
| ID: mdl-25863174
ABSTRACT
Phosphatidic acid (PA) is an abundant negatively-charged phospholipid and has long been considered to be an important signaling molecule in diverse cellular events. Thus, the identification of proteins that specifically interact with PA is of considerable interest to understand the regulatory roles of PA. Herein, lipid-affinity purification and mass spectrometric analysis reveals 43 proteins, 19 known and 24 novel, as PA-binding proteins. A lipid-protein overlay assay confirmed that GDI1, PACSIN1, and DPYSL2 interact with not only with PA but also with other phospholipids. These results might be helpful for deciphering the functional effect of PA in the brain.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Fosfatídicos
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Encéfalo
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Proteína Quinase C
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Proteínas de Transporte
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Inibidores de Dissociação do Nucleotídeo Guanina
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Peptídeos e Proteínas de Sinalização Intercelular
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Peptídeos e Proteínas de Sinalização Intracelular
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Proteínas de Membrana
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Proteínas do Tecido Nervoso
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Ano de publicação:
2015
Tipo de documento:
Article