[Investigate the clonal origin of different phenotypic tumor-like cells in the epidermis and dermis of skin lesions in lymphomatoid papulosis].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 46(2): 185-90, 2015 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-25924426
ABSTRACT
OBJECTIVE:
To explore the correlation of the clone sources of MF-like cells in the epidermis and RS-like cells in the dermis of lymphomatoid papulosis (LyP), a compartive study of this two type cells in 8 LyP cases was carried out.METHODS:
8 cases of LyP (4 type A cases and 4 type C cases) were detected the immunophenotype first, and then comparative study between the MF-like cells and RS-like cells was conducted on T-cell receptor (TCR) gamma-chain gene rearrangement by combinating laser capture microdissectinon (LCM) and PCR. PCR products of one patient which had positive bands were selected randomly for sequencing and sequence alignment analysis.RESULTS:
Monoclonal TCRgamma gene rearrangement was detected in 6 cases (75%) of the 8 cases. Detection rates of LyP type A and type C were 50% (2/4) and 100% (4/4), respectively. The rates of MF-like cells and RS-like cells were 75% (6/8) and 75% (6/8), respectively. Homology of the products with positive stripes in both MF-like cells and RS-like cells in one randomly selected case was compared and analyzed, and the results showed the homology of them was 106/108 (98%).CONCLUSION:
The MF-like cells in the epidermis and the RS-like cells in the dermis of LyP may be from the same clone.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papulose Linfomatoide
/
Derme
/
Células Epidérmicas
Limite:
Humans
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Ano de publicação:
2015
Tipo de documento:
Article