Temperature Effects on Kinetic Parameters and Substrate Affinity of Cel7A Cellobiohydrolases.

Sørensen, Trine Holst; Cruys-Bagger, Nicolaj; Windahl, Michael Skovbo; Badino, Silke Flindt; Borch, Kim; Westh, Peter

Sørensen, Trine Holst; Cruys-Bagger, Nicolaj; Windahl, Michael Skovbo; Badino, Silke Flindt; Borch, Kim; Westh, Peter.

Afiliação

Sørensen TH; From Roskilde University, Nature, Systems, and Models, Research Unit for Functional Biomaterials, 1 Universitetsvej, Building 28, DK-4000 Roskilde, Denmark and.
Cruys-Bagger N; From Roskilde University, Nature, Systems, and Models, Research Unit for Functional Biomaterials, 1 Universitetsvej, Building 28, DK-4000 Roskilde, Denmark and.
Windahl MS; From Roskilde University, Nature, Systems, and Models, Research Unit for Functional Biomaterials, 1 Universitetsvej, Building 28, DK-4000 Roskilde, Denmark and Novozymes A/S, Krogshøjvej 36, DK-2880 Bagsværd, Denmark.
Badino SF; From Roskilde University, Nature, Systems, and Models, Research Unit for Functional Biomaterials, 1 Universitetsvej, Building 28, DK-4000 Roskilde, Denmark and Novozymes A/S, Krogshøjvej 36, DK-2880 Bagsværd, Denmark.
Borch K; Novozymes A/S, Krogshøjvej 36, DK-2880 Bagsværd, Denmark.
Westh P; From Roskilde University, Nature, Systems, and Models, Research Unit for Functional Biomaterials, 1 Universitetsvej, Building 28, DK-4000 Roskilde, Denmark and pwesth@ruc.dk.

J Biol Chem ; 290(36): 22193-202, 2015 Sep 04.

Article em En | MEDLINE | ID: mdl-26183777

Assuntos

Celulose 1,4-beta-Celobiosidase/metabolismo; Celulose/metabolismo; Proteínas Fúngicas/metabolismo; Temperatura; Algoritmos; Ascomicetos/enzimologia; Ascomicetos/genética; Ligação Competitiva; Celobiose/metabolismo; Celulose 1,4-beta-Celobiosidase/genética; Eletroforese em Gel de Poliacrilamida; Proteínas Fúngicas/genética; Variação Genética; Hidrólise; Hypocrea/enzimologia; Hypocrea/genética; Isoenzimas/genética; Isoenzimas/metabolismo; Cinética; Ligação Proteica; Especificidade por Substrato; Trichoderma/enzimologia; Trichoderma/genética

Palavras-chave

Hypocrea jecorina; Rasamsonia emersonii; carbohydrate-binding protein; cellulase; enzyme kinetics; thermoactivation; thermophile

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Temperatura / Proteínas Fúngicas / Celulose / Celulose 1,4-beta-Celobiosidase Idioma: En Revista: J Biol Chem Ano de publicação: 2015 Tipo de documento: Article

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