Vasopressin inhibits mitogen-activated protein kinases and activated protein-1 in macrophages.
Acta Anaesthesiol Taiwan
; 53(3): 81-4, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-26210797
ABSTRACT
OBJECTIVES:
We have previously shown that vasopressin could inhibit the upregulation of inflammatory mediators. Expression of inflammatory mediators is tightly regulated by the upstream transcriptional pathway mitogen-activated protein kinases (MAPKs) and activated protein-1 (AP-1). In this study, we elucidated whether vasopressin could inhibit the upregulation of MAPKs/AP-1.METHODS:
Murine macrophages (RAW264.7 cells) randomly received lipopolysaccharide (LPS; 100 ng/mL) or LPS plus vasopressin (1000 pg/mL) (designated as the LPS and the LPS+V groups, respectively). Control groups were run simultaneously. For MAPKs, cells were harvested at 0 minutes, 15 minutes, 30 minutes, 45 minutes, and 60 minutes after reaction. For AP-1, cells were harvested at 60 minutes after reaction. Between-group differences in MAPKs (i.e., extracellular regulated kinase, c-Jun N-terminal kinase, and p38 MAPK) and AP-1 expressions were compared.RESULTS:
Immunoblotting assay data revealed that extracellular regulated kinase concentrations of the LPS+V group that harvested at 45 minutes and 60 minutes, but not at 15 minutes and 30 minutes, were significantly lower than those of the LPS group (p=0.005 and p=0.013). C-Jun N-terminal kinase concentrations of the LPS+V group that harvested at 15 minutes, 30 minutes, 45 minutes, and 60 minutes were also significantly lower than those of the LPS group (all p<0.001). Concentrations of p38 MAPK of the LPS+V group that harvested at 15 minutes, 30 minutes, and 45 minutes, but not at 60 minutes, were also significantly lower than those of the LPS group (all p<0.001). In addition, immunohistochemistry assay revealed that the AP-1 fluorescence signals of the LPS+V group were weaker than those of the LPS group.CONCLUSION:
Vasopressin inhibits MAPKs and AP-1 in endotoxin-activated macrophages.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasopressinas
/
Fator de Transcrição AP-1
/
Proteínas Quinases Ativadas por Mitógeno
/
Macrófagos
Limite:
Animals
Idioma:
En
Revista:
Acta Anaesthesiol Taiwan
Assunto da revista:
ANESTESIOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Taiwan