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Investigating the potential immune role of fish NCAMs: Molecular cloning and expression analysis in mandarin fish.
Wu, Nan; Zhang, Xiang-Yang; Huang, Bei; Zhang, Nu; Zhang, Xu-Jie; Guo, Xia; Chen, Xiao-Ling; Zhang, Yu; Wu, Han; Li, Shun; Li, Ai-Hua; Zhang, Yong-An.
Afiliação
  • Wu N; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Zhang XY; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Huang B; College of Fisheries, Jimei University, Xiamen 361021, China.
  • Zhang N; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Zhang XJ; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Shanghai Ocean University, Shanghai 201306, China.
  • Guo X; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 4302231, China.
  • Chen XL; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Zhang Y; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Wu H; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Li S; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Li AH; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Zhang YA; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: yzhang@ihb.ac.cn.
Fish Shellfish Immunol ; 46(2): 765-77, 2015 Oct.
Article em En | MEDLINE | ID: mdl-26277647
The immune role of NCAMs has been revealed in mammals, yet there is no such report in fish. Hence, we analyzed the molecular characterizations and immune-associated expression patterns of NCAMs in mandarin fish. Three NCAM members, named mfNCAM1a, mfNCAM1b and mfNCAM2, were identified. Among the cDNA sequences of mfNCAMs, AU-rich elements in the 3' UTRs of mfNCAM1b and mfNCAM2 as well as VASE sequences in the fourth Ig-like domain-encoding regions of mfNCAM1a and mfNCAM1b were discovered. Moreover, the syntenic analysis suggested that the duplication of NCAM1 is fish-specific. At mRNA and protein levels, the expression analyses revealed that mfNCAMs existed in both systemic and mucosal immune tissues, and located within lymphoid cells. Upon stimulated either by LPS or poly I:C, the expression level of mfNCAM1a was significantly up-regulated in head kidney, spleen, liver, and gut, whereas mfNCAM1b only in head kidney and liver, and mfNCAM2 only in liver. Additionally, the cells coexpressed mfNCAM1 and mfNCCRP-1 might imply the equivalents to mammalian NK cells. Our finding firstly demonstrates the member-specific immune-related tissue expression pattern and immune activity for fish NCAMs. Current data indicate that mfNCAM2 has little immune activity, while the immune activity of mfNCAM1a exists in more tissues than mfNCAM1b, and mfNCAM1a may tend to respond more actively to viral while mfNCAM1b to bacterial stimulants. Additionally, NCAM1b should be a fish-specific member with unique immune function, judging from its different expression pattern, immune activity as well as phylogenetic relationship to mfNCAM1a.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Perciformes / Moléculas de Adesão de Célula Nervosa / Proteínas de Peixes Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Fish Shellfish Immunol Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Perciformes / Moléculas de Adesão de Célula Nervosa / Proteínas de Peixes Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Fish Shellfish Immunol Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China