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Age-related NADPH Oxidase (arNOX) Activity Correlated with Cartilage Degradation and Bony Changes in Age-related Osteoarthritis.
Kim, Min-Jung; Kim, Hyun-Je; Hong, Young-Hoon; Lee, Choong-Ki; Kim, Yong-Woon; Shon, Oog-Jin; Song, In-Hwan.
Afiliação
  • Kim MJ; Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu, Korea.
  • Kim HJ; Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu, Korea.
  • Hong YH; Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu, Korea.
  • Lee CK; Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu, Korea.
  • Kim YW; Department of Physiology, College of Medicine, Yeungnam University, Daegu, Korea.
  • Shon OJ; Department of Orthopedic Surgery, College of Medicine, Yeungnam University, Daegu, Korea.
  • Song IH; Department of Anatomy, College of Medicine, Yeungnam University, Daegu, Korea.
J Korean Med Sci ; 30(9): 1246-52, 2015 Sep.
Article em En | MEDLINE | ID: mdl-26339163
The purpose of this study was to investigate the age-related NADPH oxidase (arNOX) activity in patients with age-related knee osteoarthritis (OA). Serum and cartilage arNOX activities were determined using an oxidized ferricytochrome C reduction assay. Full-thickness knee joint cartilages obtained through total knee replacement surgery were graded according to the Outerbridge (OB) classification. Radiographic severity of OA was determined on Knee X-rays according to the Kellgren-Lawrence (K/L) grading system. Cartilage ß-galactosidase, HIF-1α, and GLUT-1 expression levels were evaluated as markers for tissue senescence, hypoxia, and glycolysis. Higher arNOX activities occurred with higher levels of cartilage ß-galactosidase, HIF-1α, and GLUT-1 (P = 0.002). arNOX activity in cartilages with surface defects (OB grade II, III) was higher than in those without the defects (OB grade 0, I) (P = 0.012). Cartilage arNOX activity showed a positive correlation with serum arNOX activity (r = -0.577, P = 0.023). Serum arNOX activity was significantly higher in the OA subgroup with bilateral ROA than in the OA with no or unilateral ROA (2.449 ± 0.81, 2.022 ± 0.251 nM/mL, respectively, P = 0.019). The results of this study demonstrate that OA itself is not a cause to increase arNOX activities, however, arNOX hyperactivity is related to a high degree of cartilage degradation, and a high grade and extent of ROA in age-related OA.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoporose / Cartilagem Articular / Doenças das Cartilagens / Osteoartrite do Joelho Tipo de estudo: Diagnostic_studies Limite: Female / Humans / Male / Middle aged Idioma: En Revista: J Korean Med Sci Assunto da revista: MEDICINA Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoporose / Cartilagem Articular / Doenças das Cartilagens / Osteoartrite do Joelho Tipo de estudo: Diagnostic_studies Limite: Female / Humans / Male / Middle aged Idioma: En Revista: J Korean Med Sci Assunto da revista: MEDICINA Ano de publicação: 2015 Tipo de documento: Article