Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings.
J Vis Exp
; (105): e52581, 2015 Oct 25.
Article
em En
| MEDLINE
| ID: mdl-26555084
ABSTRACT
The chronically instrumented pregnant sheep has been used as a model of human fetal development and responses to pathophysiologic stimuli such as endotoxins, bacteria, umbilical cord occlusions, hypoxia and various pharmacological treatments. The life-saving clinical practices of glucocorticoid treatment in fetuses at risk of premature birth and the therapeutic hypothermia have been developed in this model. This is due to the unique amenability of the non-anesthetized fetal sheep to the surgical placement and maintenance of catheters and electrodes, allowing repetitive blood sampling, substance injection, recording of bioelectrical activity, application of electric stimulation and in vivo organ imaging. Here we describe the surgical instrumentation procedure required to achieve a stable chronically instrumented non-anesthetized fetal sheep model including characterization of the post-operative recovery from blood gas, metabolic and inflammation standpoints.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ovinos
/
Embriologia
/
Modelos Animais
/
Desenvolvimento Fetal
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
J Vis Exp
Ano de publicação:
2015
Tipo de documento:
Article