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Transcriptomic and epigenomic characterization of the developing bat wing.
Eckalbar, Walter L; Schlebusch, Stephen A; Mason, Mandy K; Gill, Zoe; Parker, Ash V; Booker, Betty M; Nishizaki, Sierra; Muswamba-Nday, Christiane; Terhune, Elizabeth; Nevonen, Kimberly A; Makki, Nadja; Friedrich, Tara; VanderMeer, Julia E; Pollard, Katherine S; Carbone, Lucia; Wall, Jeff D; Illing, Nicola; Ahituv, Nadav.
Afiliação
  • Eckalbar WL; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
  • Schlebusch SA; Institute for Human Genetics, University of California, San Francisco, San Francisco, California, USA.
  • Mason MK; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa.
  • Gill Z; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa.
  • Parker AV; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa.
  • Booker BM; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa.
  • Nishizaki S; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
  • Muswamba-Nday C; Institute for Human Genetics, University of California, San Francisco, San Francisco, California, USA.
  • Terhune E; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
  • Nevonen KA; Institute for Human Genetics, University of California, San Francisco, San Francisco, California, USA.
  • Makki N; Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa.
  • Friedrich T; Oregon National Primate Research Center, Division of Neuroscience, Primate Genetics Section, Beaverton, Oregon, USA.
  • VanderMeer JE; Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado, USA.
  • Pollard KS; Oregon National Primate Research Center, Division of Neuroscience, Primate Genetics Section, Beaverton, Oregon, USA.
  • Carbone L; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
  • Wall JD; Institute for Human Genetics, University of California, San Francisco, San Francisco, California, USA.
  • Illing N; Institute for Human Genetics, University of California, San Francisco, San Francisco, California, USA.
  • Ahituv N; Gladstone Institutes, San Francisco, California, USA.
Nat Genet ; 48(5): 528-36, 2016 05.
Article em En | MEDLINE | ID: mdl-27019111
ABSTRACT
Bats are the only mammals capable of powered flight, but little is known about the genetic determinants that shape their wings. Here we generated a genome for Miniopterus natalensis and performed RNA-seq and ChIP-seq (H3K27ac and H3K27me3) analyses on its developing forelimb and hindlimb autopods at sequential embryonic stages to decipher the molecular events that underlie bat wing development. Over 7,000 genes and several long noncoding RNAs, including Tbx5-as1 and Hottip, were differentially expressed between forelimb and hindlimb, and across different stages. ChIP-seq analysis identified thousands of regions that are differentially modified in forelimb and hindlimb. Comparative genomics found 2,796 bat-accelerated regions within H3K27ac peaks, several of which cluster near limb-associated genes. Pathway analyses highlighted multiple ribosomal proteins and known limb patterning signaling pathways as differentially regulated and implicated increased forelimb mesenchymal condensation in differential growth. In combination, our work outlines multiple genetic components that likely contribute to bat wing formation, providing insights into this morphological innovation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Asas de Animais / Quirópteros / Epigênese Genética / Transcriptoma Limite: Animals Idioma: En Revista: Nat Genet Assunto da revista: GENETICA MEDICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Asas de Animais / Quirópteros / Epigênese Genética / Transcriptoma Limite: Animals Idioma: En Revista: Nat Genet Assunto da revista: GENETICA MEDICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos