Generating hESCs with reduced immunogenicity by disrupting TAP1 or TAPBP.
Biosci Biotechnol Biochem
; 80(8): 1484-91, 2016 Aug.
Article
em En
| MEDLINE
| ID: mdl-27068360
ABSTRACT
Human embryonic stem cells (hESCs) are thought to be a promising resource for cell therapy, while it has to face the major problem of graft immunological rejection. Major histocompatibility complex (MHC) class I expressed on the cell surface is the major cause of graft rejection. Transporter associated with antigen presentation 1 (TAP1) and TAP-associated glycoprotein (TAPBP) play important roles in regulating MHC class I expression. In this study, we generated TAP1- and TAPBP-deficient hESC lines, respectively, using transcription activator-like effector nucleases technique. These cells showed deficient expression of MHC class I on the cell surface and reduced immunogenicity compared with wild types, but maintained normal pluripotency, karyotypes, and differentiation ability. Thus, our findings are instrumental in developing a universal cell resource with both pluripotency and hypo-immunogenicity for transplantation therapy in the future.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
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Antígenos de Histocompatibilidade Classe I
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Células-Tronco Pluripotentes
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Transplante de Células-Tronco
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Células-Tronco Embrionárias Humanas
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Membro 2 da Subfamília B de Transportadores de Cassetes de Ligação de ATP
Limite:
Animals
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Humans
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Assunto da revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Ano de publicação:
2016
Tipo de documento:
Article