RNA fingerprinting of respiratory syncytial virus using ribonuclease protection. Application to molecular epidemiology.
J Clin Invest
; 83(6): 1894-902, 1989 Jun.
Article
em En
| MEDLINE
| ID: mdl-2723064
ABSTRACT
We have used the technique of ribonuclease protection to define genomic variation among circulating isolates of subgroup A respiratory syncytial (RS) virus. RNAs extracted from HEp-2 cells infected with strains to be analyzed were hybridized with a 32P-labeled RNA probe corresponding to the RS virus G glycoprotein (A2 strain). Areas of nonhomology were detected by cleavage with ribonuclease A. Using this technique, multiple distinct RNA cleavage patterns could be distinguished among viral isolates recovered from infants residing in the same metropolitan area and infected during the same epidemic season. Epidemiologically related isolates (from coinfected twins, from infants infected during a nosocomial outbreak at an extended care facility, and from institutionalized adults infected during an outbreak) yielded identical patterns. In two separate outbreaks, differences in cleavage patterns among certain isolates corresponded to epidemiologically significant differences among the individuals from whom the isolates were recovered. We conclude that substantial genomic heterogeneity exists among circulating isolates of subgroup A RS virus. Ribonuclease protection can be used as a molecular fingerprinting tool for expanded studies of the molecular epidemiology of this virus.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Respirovirus
/
Vírus Sinciciais Respiratórios
/
Ribonucleases
/
Mapeamento de Nucleotídeos
/
RNA Viral
/
Sondas RNA
Tipo de estudo:
Screening_studies
Limite:
Adult
/
Humans
/
Infant
País/Região como assunto:
America do norte
Idioma:
En
Revista:
J Clin Invest
Ano de publicação:
1989
Tipo de documento:
Article