A quantitative headspace-solid-phase microextraction-gas chromatography-flame ionization detector method to analyze short chain free fatty acids in rat feces.
Anal Biochem
; 508: 12-4, 2016 09 01.
Article
em En
| MEDLINE
| ID: mdl-27267560
ABSTRACT
This study sought to develop and validate a quantitative method to analyze short chain free fatty acids (SCFAs) in rat feces by solid-phase microextraction and gas chromatography (SPME-GC) using the salt mixture ammonium sulfate and sodium dihydrogen phosphate as salting out agent. Conditioning and extraction time, linearity, limits of detection and quantification, repeatability, and recovery were evaluated. The proposed method allows quantification with improved sensitivity as compared with other methods exploiting SPME-GC. The method has been applied to analyze rat fecal samples, quantifying acetic, propionic, isobutyric, butyric, isopentanoic, pentanoic, and hexanoic acids.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Química Analítica
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Cromatografia Gasosa
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Microextração em Fase Sólida
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Ácidos Graxos não Esterificados
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Fezes
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Ionização de Chama
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2016
Tipo de documento:
Article