Whole cell-based surface plasmon resonance measurement to assess binding of anti-TNF agents to transmembrane target.
Anal Biochem
; 508: 73-7, 2016 09 01.
Article
em En
| MEDLINE
| ID: mdl-27349512
ABSTRACT
We developed a technique for the measurement of surface plasmon resonance (SPR) to detect interactions of anti-tumor necrosis factor (TNF) agents with transmembrane TNF-α (mTNF-α) on living whole cells. The injection of a suspension of mTNF-α expressing Jurkat cells, used as an analyte, gave a clear binding response to anti-TNF agents, such as etanercept, infliximab and adalimumab, immobilized on sensorchip. The binding response of the analyte cells increased in a concentration-dependent manner and was competitively reduced by adding soluble TNF receptors to the analyte cell suspension. Treatment of analyte cells with free anti-TNF agent before injection reduced the binding response between the analyte cells and immobilized-etanercept on sensorchip, and the inhibitory effect of free anti-TNF agent was concordant with the affinity of anti-TNF agent for soluble TNF-α. These findings indicate that the SPR response arises from specific binding between anti-TNF agent and its target on cell membrane.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
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Técnicas de Química Analítica
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Fator de Necrose Tumoral alfa
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Antirreumáticos
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Ressonância de Plasmônio de Superfície
Limite:
Humans
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2016
Tipo de documento:
Article