Efficient production of (2)H, (13)C, (15)N-enriched industrial enzyme Rhizopus chinensis lipase with native disulfide bonds.

Zhang, Meng; Yu, Xiao-Wei; Swapna, G V T; Xiao, Rong; Zheng, Haiyan; Sha, Chong; Xu, Yan; Montelione, Gaetano T

Zhang, Meng; Yu, Xiao-Wei; Swapna, G V T; Xiao, Rong; Zheng, Haiyan; Sha, Chong; Xu, Yan; Montelione, Gaetano T.

Afiliação

Zhang M; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China.
Yu XW; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China. bioyuxw@aliyun.com.
Swapna GV; State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China. bioyuxw@aliyun.com.
Xiao R; Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
Zheng H; Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
Sha C; Northeast Structural Genomics Consortium, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
Xu Y; Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
Montelione GT; Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.

Microb Cell Fact ; 15(1): 123, 2016 Jul 13.

Article em En | MEDLINE | ID: mdl-27411547

Assuntos

Lipase/química; Lipase/metabolismo; Rhizopus/enzimologia; Isótopos de Carbono/metabolismo; Deutério/metabolismo; Dissulfetos/química; Dissulfetos/metabolismo; Escherichia coli/genética; Escherichia coli/metabolismo; Glicosilação; Marcação por Isótopo; Lipase/genética; Isótopos de Nitrogênio/metabolismo; Pichia/genética; Pichia/metabolismo; Dobramento de Proteína; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Rhizopus/química

Palavras-chave

2H, 13C, 15N-enriched protein production; Native disulfide bonds; Nuclear magnetic resonance spectroscopy; Rhizopus chinensis lipase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rhizopus / Lipase Idioma: En Revista: Microb Cell Fact Assunto da revista: BIOTECNOLOGIA / MICROBIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China

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